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Biochemical tests are most often applied to samples of serum, plasma and urine where levels of specific chemicals are measured and the results compared with those representative of a healthy individual. An increase or decrease in any particular component(s) can help to identify a disease process.

Biochemical tests are used to identify bacterial species by differentiating them on the basis of biochemical activities. The difference in protein and fat metabolism, carbohydrate metabolism, enzyme production, compound utilization ability, etc. are some factors that aid in bacterial identification.